This video contains an overview of ABE's Lab 5/5A: "Transforming Bacteria with the Ligation Products". Once a recombinant plasmid that includes the gene of interest has been created, the next step is to replicate the plasmid and allow the bacteria to produce the protein. 

In this lab, students carry out the transformation of E. coli bacteria using a recombinant plasmid that contains the rfp gene. If transformation was successful, bacteria will glow red because they are producing the red fluorescent protein.