This PDF file contains all of the files pertaining to Lab 2A.

This PDF file contains all of the files pertaining to Lab 4A.

This video contains an overview of ABE's Lab 5/5A: "Transforming Bacteria with the Ligation Products". Once a recombinant plasmid that includes the gene of interest has been created, the next step is to replicate the plasmid and allow the bacteria to produce the protein. 

In this lab, students carry out the transformation of E. coli bacteria using a recombinant plasmid that contains the rfp gene. If transformation was successful, bacteria will glow red because they are producing the red fluorescent protein.

This PDF file contains all of the files pertaining to Lab 5A.

This video provides a detailed overview of ABE's Lab 6: Purifying the Fluorescent Protein. In the first part of this laboratory, students use a reagent called “lysis buffer” to lyse (break open) the cells. In the second part of this laboratory, students use column chromatography to separate out the red fluorescent protein from the sample. The properties of the protein’s amino acids allow students to separate it from the rest of the sample using column chromatography. When students pass the sample through the column, the hydrophobic red fluorescent protein will stick to the resin beads, while the hydrophilic amino acids will pass through. Students will then wash out the remaining somewhat-hydrophobic proteins in the sample using a salt buffer solution. Finally, students will use another buffer solution to release the bound red fluorescent protein from the column and collect the product. This is the same process that would be used to isolate a human therapeutic protein.

This PDF file contains all of the files pertaining to Lab 6.

Directions to grow bacteria for protein purification (Lab 6).

adopted from Alia Qatarneh, ABE MA director

Overview with links

This video reviews key content for Lab 1: Tools of the Trade. This lab introduces students to some tools of genetic engineering such as micropipetting and gel electrophoresis. It gives students hands-on experience with some important tools and techniques commonly used in molecular biology.