This short refresher video (2:23) walks teachers through the essential steps for making an agarose gel for ABE electrophoresis. It’s designed as a quick memory‑jog for anyone who hasn’t cast gels in a while.

What the video covers

• Preparing the agarose solution The video reminds you how to measure and mix agarose with buffer before heating.
• Heating and dissolving the agarose It shows the typical process of melting agarose until fully clear.
• Setting up the casting tray The tray and comb are positioned correctly so the wells form at the right end.
• Pouring the gel The molten agarose is poured smoothly into the tray, avoiding bubbles.
• Letting the gel set The gel is left to solidify before removing the comb and using it in the MiniOne system.

Important update noted in the description

The video includes a reminder that ABE UK now uses 1× TAE buffer instead of 1× SB buffer, which is a change from when the video was originally recorded.