Aliquoting Guide for Lab 3

We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.

Label tube Contents of tube Aliquot Actually used
5xB 5x ligation buffer 4µL 3µL
**LIG T4 DNA ligase 2µL 2µL
*dH2O Distilled water 1000µL 2µL

**NOTE: Students work directly in the LIG tube, do not aliquot any extra.
*dH2O is used for several labs.


Resource category
ABE Lab Specific Resources
Related Lab Lab 3: Building a Recombinant Plasmid