DNA fingerprinting SIBE Japanese knotweed

DNA fingerprinting SIBE orangutan parentage

DNA fingerprinting SIBE otter

DNA fingerprinting SIBE reforestation project

DNA Markers (1 Kb) H&S information

Labs 5, 7.2, 9.2

Can be used alone for simple electrophoresis
 

DNA Markers (100 bp) H&S information

Lab 7.2

Can be used alone for simple electrophoresis, or Lab 8
 

THE BIG IDEA
DNA -> Protein -> Trait

Flowchart - The Big Idea overview of labs and relatedness

Tools of the trade - using a micropipette

Tools of the trade - gel electrophoresis

Beginning to clone a gene - restriction digestion of plasmids

Building a recombinant plasmid - building the pARA-R plasmid

Checking you've created a recombinant plasmid - preparation of samples for agarose gel electrophoresis

Inserting recombinant plasmids into bacteria - bacterial transformation using ligation mix

Inserting recombinant plasmids into bacteria - bacterial transformation using pARA-R

As this animation shows, gel electrophoresis enables scientists to determine the size of DNA molecules.

Gel green H&S information -MiniOne only

Labs 5, 7,2, 9.2

Always added to samples before MO electrophoresis
 

Gel red H&S information

Labs 5, 7.2, 9.2

Gene editing CRISPR Cas9 advances and challenges part 2

Gene editing CRISPR Cas9 advances and challenges PowerPoint  part 1