Restriction Enzyme - BamHI High Fidelity H&S information

Labs 1.2, 9.1

Restriction Enzyme - EcoRI  H&S information

Lab 9.1

Restriction Enzyme - HindIII High Fidelity H&S information

Labs 2, 9.1

Restriction Enzyme - PstI H&S information

Lab 9.1

Restriction Enzyme - PvuII High Fidelity H&S information

Lab 9.1

Short video by Katie Porter describing restriction enzymes

Restriction map prediction PowerPoint

Restriction mapping exercise plan

Restriction mapping student sheets

A short film showing the procedures involved in the production and running of an agarose gel.

The film was produced by GENIE, based within the Genetics Department at the University of Leicester. Further information and materials can be found on the GENIE web site http://www.le.ac.uk/ge/genie/ and the GENIE VGEC web site http://www.le.ac.uk/ge/genie/vgec/

T4 DNA ligase H&S information

Lab 3

T4 DNA ligase Quick ligation reaction buffer H&S information

Lab 3

T4 DNA ligase reaction buffer H&S information

Lab 3

TAE buffer 50x H&S information
Labs 5, 7.2, 9.2

TAE diluted to x1 in the electrophoresis labs
 

Taq 2x master mix M0270 H&S information

Labs 4, 7.1

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Tris buffer solution H&S information

Labs 1.1, 1.2, 5, 7.2, 9.2

Anything involving S1, S2 or S3 (dye solutions) or Loading Dye
 

Xylene Cyanol FF H&S information

Labs 1.1, 1.2, 5, 7.2, 9.2

# Loading Dye (LD) is a mixture of the 3 dyes Bromophenol Blue, Xylene Cyanol and Orange G and is used in any electrophoresis