Aliquoting Guide for Lab 2

We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.

Label tube Contents of tube Aliquot Actually used
A pARA (80ng/µL) 10µL 8µL
K pKAN-R (80ng/µL) 10µL 8µL
RE BamH I and Hind III 5µL 4µL
2.5xB 2.5x restriction buffer 20µL 16µL
*dH2O Distilled water 1000µL 4µL

*dH2O is used for several labs.

Resource category
ABE Lab Specific Resources
Related Lab Lab 2: Preparing to Clone a Gene