Aliquoting Guide for Lab 4

We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.

Label tube Contents of tube Aliquot Actually used
M 1 kb ladder 10µL 10µL
LD Loading dye 12µL 10µL
*dH2O Distilled water 1000µL 19µL

*dH2O is used for several labs.

Reminder: The ladder already contains loading dye. Also, Solution 2 (Lab 1) is the same as loading dye.


Resource category
ABE Lab Specific Resources
Related Lab Lab 4: Verifying the Recombinant Plasmid